
PMK562M PMK562S

50ul 100ul

WB, IHC

Recombinant Strep Tag II Specific

Strep Tag II

IgG1

PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.Store at -20°C.
Do not aliquot the antibody.

WB: 1:5,000 IHC: 1:200

The Strep Tag II Mouse Monoclonal Antibody can detects Strep Tag II recombinant protein.

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide used in numerous molecular biological
protocols dues to its strong affinity for biotin. NWSHPQFEK (Strep tag II) is a nine amino acid peptide with high specificity
and affinity towards streptavidin. NWSHPQFEK Tag Antibody is a useful tool in analysis and affinity purification of Strep tag
II fusion proteins.

Flag-Tag Mouse Monoclonal Antibody(2C5)
(PMK001M /PMK001S)
HA Mouse Monoclonal Antibody(1B10)-HRP conjugated
(PMK061M /PMK061S)
His-Tag Rabbit Polyclonal Antibody
(PMK111M /PMK111S)
C-Myc-Tag Mouse Monoclonal Antibody(3E8)
(PMK012M /PMK012S)
GAPDH Mouse Monoclonal Antibody(2B8)-HRP conjugated
(PMK053M /PMK053S)
Lamin B1 Mouse Monoclonal Antibody(7C11)
(PMK049C /PMK049F)
β-actin Mouse Monoclonal Antibody(5B7)-HRP conjugated
(PMK058M /PMK058S)
Rabbit anti-Goat IgG (H+L), HRP Conjugated
(PMK-014-097M /PMK-014-097S)

WB-Western blot
IHC-Immunochemistry
IF-Immunofluorescence
IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation

H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog
Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep Pg-Pig
更新中...
答:可能的原因有:
1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;
3、杂蛋白多,建议处理目的蛋白;
4、抗体特异性不强,建议使用特异性强的抗体;
5、抗体孵育时间过久,建议减少抗体孵育时间;
6、二抗与抗原有交叉反应,建议选择合适的封闭物;
7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;
8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。
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