
Storage Buffer : 0.1M Sodium phosphate, 0.15M NaCl, 50% glycerol, pH7.4
Stabilizer: 1% Bovine Serum Albumin (IgG-Free,Protease-Free)
Preservative: 0.02% Sodium Azide
Suggested Dilution Range: 1:100-1:800 for most applications
Optimal dilutions should be determined by the end user.
Fluorophore: Dylight 680--TFP ester
Amax=682nm; Emax=715nm
Purity:The antibody reacts with whole molecule mouse IgG. It also reacts with the light chains of other mouse immunoglobulins.
Antibody Source:The antibody was affinity purified from antisera by immunoaffinity chromatography using purified mouse IgG.
Expiration date:Store at -20°C. Avoid repeated freeze / thaw cycles.The expiration date may be extended if test results are acceptable for the intended use.
更新中...
答:可能的原因有:
1、细胞中不表达这种蛋白质,换一种细胞;
2、抗体不能识别目标蛋白,多看看说明,是否有问题;
3、可能是没有保持低温操作,样品保存不当,样品放置时间过长;
4、细胞中的蛋白质被酶降解掉了,可加入蛋白酶抑制剂,抑制蛋白酶活性。
答:一般5×10^6就足够。
1、可以加大抗原上样量,这是主要的;
2、也可以将一抗稀释比例降低;
3、还可以延长曝光时间。
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