特超敏ECL发光底物检测试剂盒(低阿克级)
货号:PMK003
一键复制产品信息
价格:
580.00
规格:
100ml
500ml
货期:
现货
本产品4 ℃运输,保存于4℃,保质期 12个月。
Details
产品详情
  • 产品简介

    特超敏ECL发光底物检测试剂盒用于检测直接或间接标记辣根过氧化物酶HRP的抗体及其关联的抗原,是一种可广泛应用于免疫学实验的化学发光试剂。由于采用了独特的发光底物系统和高灵敏度荧光ECL发光试剂,特超敏ECL发光底物检测试剂盒是目前灵敏的商业化荧光ECL检测试剂之一,灵敏度高于常规超敏ECL检测试剂数十倍乃至数百倍:

    1、具有极高灵敏度和高信噪比,可检测10~100fg微量抗原;

    2、发光迅速且稳定,荧光可使X光胶片感光达12小时以上,特别适用于痕量蛋白或核酸检测;

    3、可使用更高的抗体稀释倍数(1:5000~1:20000),极其节省抗体。

    4、优异的性价比,更高的性能,更低的价格。

  • 注意事项

    1、本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

    2、为了您的安全和健康,请穿实验服并戴一次性手套操作,且戴手套可以避免在印记膜上留下手印。

Instruction manual/COA download
说明书/COA下载
Published literature
已发表文献
  • 1. Dou RX, Zhang YM, Hu XJ, Gao FL, Zhang LL, Liang YH, Zhang YY, Yao YP, Yin L, Zhang Y, Gu C. Aβ1-42 promotes microglial activation and apoptosis in the progression of AD by binding to TLR4. Redox Biol. 2024 Dec;78:103428. doi: 10.1016/j.redox.2024.103428. Epub 2024 Nov 14. PMID: 39550828; PMCID: PMC11615585. IF 10.7

    2. Li LG, Yang XX, Xu HZ, Yu TT, Li QR, Hu J, Peng XC, Han N, Xu X, Chen NN, Chen X, Tang JM, Li TF. A Dihydroartemisinin-Loaded Nanoreactor Motivates Anti-Cancer Immunotherapy by Synergy-Induced Ferroptosis to Activate Cgas/STING for Reprogramming of Macrophage. Adv Healthc Mater. 2023 Nov;12(28):e2301561. doi: 10.1002/adhm.202301561. Epub 2023 Aug 23. PMID: 37567571. IF 10

    3. Xiaotong Wang, Di Zhang, Yaxue Zhu, Daojie Li, Long Shen, Qiankun Wang, Yun Gao, Xiaoping Li, Mei Yu,Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets,Science of The Total Environment,Volume 908,2024,168303,ISSN 0048-9697,https://doi.org/10.1016/j.scitotenv.2023.168303. (IF 9.8)

    4. Xin X Y ,Xiang X ,Fang M W , et al. A nanoreactor boosts chemodynamic therapy and ferroptosis for synergistic cancer therapy using molecular amplifier dihydroartemisinin[J].Journal of Nanobiotechnology,2022,20(1):230-230. (IF 9.429)

    5. Gen L L ,Chun X P ,Ting T Y , et al.Dihydroartemisinin remodels macrophage into an M1 phenotype via ferroptosis-mediated DNA damage[J].Frontiers in Pharmacology,2022,13949835-949835. (IF 5.988)

    6. Xu Z H ,Chen X F ,Li K , et al.Anti-lung cancer synergy of low-dose doxorubicin and PD-L1 blocker co-delivered via mild photothermia-responsive black phosphorus.[J].Drug delivery and translational research,2024,15(1):1-22. (IF 5.7)

    7. Ting-Ting Y ,Jun H ,Qi-Rui L , et al.Chlorin e6-induced photodynamic effect facilitates immunogenic cell death of lung cancer as a result of oxidative endoplasmic reticulum stress and DNA damage[J].International Immunopharmacology,2023,115. (IF 5.6)

    8. Wenqi L ,Shiwen K ,Huijian C , et al.A composition of ursolic acid derivatives from Ludwigia hyssopifolia induces apoptosis in throat cancer cells via the Akt/mTOR and mitochondrial signaling pathways and by modulating endoplasmic reticulum stress.[J].Journal of ethnopharmacology,2023,319(P3):117351-117351. (IF 5.4)

    9. Xin L ,Huimin L ,Yaping F , et al.Resatorvid alleviates experimental inflammatory TMJOA by restraining chondrocyte pyroptosis and synovial inflammation[J].Arthritis Research & Therapy,2023,25(1):230-230. IF 4.9

    10. Sun Y ,Chen Y ,Zhao H , et al.Lactate-driven type I collagen deposition facilitates cancer stem cell-like phenotype of head and neck squamous cell carcinoma[J].iScience,2024,27(4):109340-. (IF 4.6)

    11. Jian Y H ,Ting T Y ,Gen L L , et al.B-Myb deficiency boosts bortezomib-induced immunogenic cell death in colorectal cancer[J].Scientific Reports,2024,14(1):7733-7733. (IF 3.8)

    12. Li C ,Liu W ,Liu Y , et al.Role of ATP citrate lyase and its complementary partner on fatty acid synthesis in gastric cancer.[J].Scientific reports,2024,14(1):30043. (IF 3.8)

    13. YuanJian H ,Hao C ,XingChun P , et al.Up-regulation of ABCG2 by MYBL2 deletion drives Chlorin e6-mediated photodynamic therapy resistance in colorectal cancer.[J].Photodiagnosis and photodynamic therapy,2023,42103558-103558. (IF 3.3)

    14. ZiYi Y ,LiuGen L ,YiLian X , et al.Cepharanthine synergizes with photodynamic therapy for boosting ROS-driven DNA damage and suppressing MTH1 as a potential anti-cancer strategy.[J].Photodiagnosis and photodynamic therapy,2023,45103917-103917. (IF 3.3)

    15. QiRui L ,HuaZhen X ,RongCheng X , et al.Laser-triggered intelligent drug delivery and anti-cancer photodynamic therapy using platelets as the vehicle.[J].Platelets,2023,34(1):2166677-2166677. (IF 3.3)

    16. Quan W ,Xu S C ,Ma C , et al.Anti-tumor effects of telmisartan in glioma-astrocyte non-contact co-cultures: A critical role of astrocytic IL-6-mediated paracrine growth promotion[J].International Immunopharmacology,2024,139112707-112707. (IF 4.8)

    17. Li G L ,Zhang D ,Huang Q , et al.Mitochondrial disruption resulting from Cepharanthine-mediated TOM inhibition triggers ferroptosis in colorectal cancer cells[J].Journal of Cancer Research and Clinical Oncology,2024,150(10):460-460. (IF 2.7)

    18. Wen H ,Yuan L ,Wei C , et al.Effects of combined exposure to formaldehyde and benzene on immune cells in the blood and spleen in Balb/c mice[J].Environmental Toxicology and Pharmacology,2016,45265-273. (IF 2.187)

    19. Wei Q ,ChengShi X ,XiaoChong L , et al. Telmisartan inhibits microglia-induced neurotoxic A1 astrocyte conversion via PPARγ-mediated NF-κB/p65 degradation.[J].International immunopharmacology,2023,123110761-110761. (IF 5.6)

    20. Chenxi W ,Huaxiao W ,Langyue Y , et al.Formaldehyde induces toxicity in mouse bone marrow and hematopoietic stem/progenitor cells and enhances benzene-induced adverse effects.[J].Archives of toxicology,2017,91(2):921-933. (IF 6.637)

    21. Liu S ,Wang Y ,Ying L , et al.Quercetin Mitigates Lysophosphatidylcholine (LPC)-Induced Neutrophil Extracellular Traps (NETs) Formation through Inhibiting the P2X7R/P38MAPK/NOX2 Pathway[J].International Journal of Molecular Sciences,2024,25(17):9411-9411. (IF 4.9)

    22. Li G ,Wu M ,Chen K , et al.ROS-mediated M1 polarization-necroptosis crosstalk involved in Di-(2-ethylhexyl) phthalate-induced chicken liver injury.[J].Poultry science,2024,104(1):104558. (IF 3.8)

    23. Yuan Z L ,Tian L ,Feng T , et al.ZDHHC15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with glioma[J].BMC Cancer,2023,23(1):420-420. (IF 3.8)

    24. Wei C ,Chen M ,You H , et al.Formaldehyde and co-exposure with benzene induce compensation of bone marrow and hematopoietic stem/progenitor cells in BALB/c mice during post-exposure period[J].Toxicology and Applied Pharmacology,2017,32436-44. (IF 3.791)

    25. Zhang J , Li Y , Wang Y , et al.Transcriptome Sequencing and Metabolite Analysis Revealed the Single and Combined Effects of Microplastics and Di-(2-ethylhexyl) Phthalate on Mouse Liver. International Journal of Molecular Sciences. 2025; 26(10):4943.  (IF 4.9)

    26.  Li LG, Chen H, Yu TT, et al. Suppression of NRF2 by photodynamic action to enhance bortezomib-triggered DNA damage for synergistic colorectal cancer therapy. J Photochem Photobiol B. 2025;267:113162. (IF 3.7)

    27. Hu X, Guo H, Wang G, et al. Poly (sodium lipoate) Particles with Nitroimidazole Modification for Disulfide Stress-Mediated Antitumor Metastasis[J]. ACS Applied Materials & Interfaces, 2025.(IF 8.2)

    28. Dai Y, Zheng Z, Ma J, et al. Protective effects of artesunate on the neurovascular unit by regulating PDGFRβ signaling in experimental cerebral malaria[J]. Biochemical Pharmacology, 2025: 117023.(IF 5.6)

    29. Wen S, Fu A, Liu F, et al. Identifying potential drugs for treating Cardiovascular-kidney metabolic syndrome via reverse network pharmacology[J]. Frontiers in Pharmacology, 2025, 16: 1627236.(IF 4.8)

    30. Yang G, Guo H, Zhou P, et al. PERK-mediated eIF2α phosphorylation suppresses porcine epidemic diarrhea virus replication by attenuating global protein synthesis and inducing IFN-Ⅰ production[J]. Veterinary Microbiology, 2025: 110559.(IF 2.7)

    31.  Yunfei Zou, Junyi Li, Changwen Xu,   Empagliflozin alleviates doxorubicin-induced myocardial injury by inhibiting RIP3-dependent TLR4/MyD88/NF-κB signaling pathway. (IF 5.6)

    32.1.Shiyuan Cheng, Huijun Li,Smilax china L. Extract Alleviates Metabolic-Associated Fatty Liver Disease by Regulating Gut Microbiota and Bile Acid Metabolism,Metabolites,2025.(IF 3.7)

    33.Zhong Shao, Ke Li,An oral H2S nanotherapeutics for hypertensive chronic kidney disease via synergistic antihypertensive and renoprotective activities,Journal of Neuroinflammation,2025.(IF 11.5)

    34.Huaxiao Wen, Langyue Yuan,Effects of combined exposure to formaldehyde and benzene on immune cells in the blood and spleen in Balb/c mice,ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2016.(IF 2.187)

Product Q&A FAQ
产品问答FAQ
  • Western Blot(WB)实验中有很多杂带咋回事?

    答:可能的原因有:

    1、目的蛋白有多个修饰位点,本身可以呈现多条带,建议查阅文献或进行生物信息学分析,获得蛋白序列的修饰位点信息,通过去修饰确定蛋白实际大小;

    2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂;样本处理时在冰上操作;

    3、杂蛋白多,建议处理目的蛋白;

    4、抗体特异性不强,建议使用特异性强的抗体;

    5、抗体孵育时间过久,建议减少抗体孵育时间;

    6、二抗与抗原有交叉反应,建议选择合适的封闭物;

    7、二聚体或多聚体存在,建议增加蛋白质变性过程及强度;

    8、底物显色与曝光时间过长,建议缩短显色及曝光的时间。


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