特超敏ECL发光底物检测试剂盒用于检测直接或间接标记辣根过氧化物酶HRP的抗体及其关联的抗原，是一种可广泛应用于免疫学实验的化学发光试剂。由于采用了独特的发光底物系统和高灵敏度荧光ECL发光试剂，特超敏ECL发光底物检测试剂盒是目前灵敏的商业化荧光ECL检测试剂之一，灵敏度高于常规超敏ECL检测试剂数十倍乃至数百倍：
1、具有极高灵敏度和高信噪比，可检测10～100fg微量抗原；
2、发光迅速且稳定，荧光可使X光胶片感光达12小时以上，特别适用于痕量蛋白或核酸检测；
3、可使用更高的抗体稀释倍数(1:5000～1:20000)，极其节省抗体。
4、优异的性价比，更高的性能，更低的价格。
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答：可能的原因有：
1、目的蛋白有多个修饰位点，本身可以呈现多条带，建议查阅文献或进行生物信息学分析，获得蛋白序列的修饰位点信息，通过去修饰确定蛋白实际大小；
2、样本处理过程中目的蛋白发生降解,建议加入蛋白酶抑制剂；样本处理时在冰上操作；
3、杂蛋白多，建议处理目的蛋白；
4、抗体特异性不强，建议使用特异性强的抗体；
5、抗体孵育时间过久，建议减少抗体孵育时间；
6、二抗与抗原有交叉反应，建议选择合适的封闭物；
7、二聚体或多聚体存在，建议增加蛋白质变性过程及强度；
8、底物显色与曝光时间过长，建议缩短显色及曝光的时间。
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